Purification Of Bioactive Compounds From Euphorbia Hirta Linn. 'S Fractional Extract For Antidiabetes Drug Development

Abstract

This study objects to purification of bioactive compounds from Euphorbia hirta Linn. for further research. Strong anti-hyperglycemic activity of Euphorbiaceae family in general and Euphorbia hirta Linn. in particular has elucidated by a lot of research. However, these previous research mainly focused on testing bioactivities with total extract or fractional extracts. An idea of isolating bioactive compounds for bioactivities testing and drug development, therefore, is still quite novel and potential. Preliminarily, STZ/NAD mice were tested for anti-hyperglycemic activity to determine which fractional extract gave significant changes in blood glucose level after oral administration with dose of 600 mg/kg body weight. In this test, acarbose was used as positive control with dose of 100 mg/kg body weight. The result indicated that chloroform extract, ethyl acetate extract and 1-buthanol extract had high anti-glycemic effect. Although ethyl acetate extract showed the greatest anti-hyperglycemic effect, chloroform extract was chosen to isolate bioactive compounds due to the limited time of the B.Sc. thesis. Column chromatography technique was applied to isolate compounds from chloroform extract paralleled with using thin layer chromatography for evaluate a number of fractions to investigate the suitable solvent system and examine whether obtained crystalline solid was pure or not. According to 1H NMR and 13C NMR spectra, crystalline solid CSCF6-1 was successfully purified. Comparing with spectral signals of previously identified compounds, spectral signal of CSCF6-1 differed from them. In this circumstance, only 1H NMR and 13C NMR spectra could not be used as strong foundation to give conclusion about its molecular structure Although CSCF6-1 structure was still unidentified, new orientations were widely opened for the next research about CSCF6-1 in future. Key words: diabetes, anti-hyperglycemic, thin layer chromatography (TLC), column chromatography (CC), nuclear magnetic resonance (NMR)