| dc.description.abstract | Tissue engineering has been focused in the past decades as a new therapy for tissue repair and regeneration. Specifically, 3D scaffold plays an important role as a type of temporary architecture to assist cell growth and tissue formation. In Vietnam, there have been only limited research and publications in this field. This study reports the fabrication of polymeric 3D scaffolds for bone tissue regeneration using emulsion freeze-drying method. Foams of poly (ɛ-caprolactone) (PCL) with different percentages of water (20% and 40%) were made, characterized and tested with bone marrow derived human mesenchymal stem cell (hBMMSC) for biocompatibility and cell proliferation. hBMMSC was successfully seeded and grown on PCL scaffolds. After 1, 3, and 7 days of culturing on scaffold, increased cell viability observed via modified MTT assay (by around 6% and 16% with 20% water scaffold and 40% scaffold accordingly) suggests the potential of applying this type of material to tissue engineering in Vietnam.
Keywords:
Polymeric 3D scaffold Poly (ɛ-caprolactone)
Emulsion freeze-drying
Human mesenchymal stem cell | en_US |
