Assessment of leuconostoc nanocell in antifungal drug delivery in mice

Abstract

Over 30 years, nanoparticle as a drug delivery is considered as one of the indispensable issues in pharmaceutical fields. Increasing the bioavailability of insoluble or slightly soluble drug and prolonging the effect of drug are important to reduce drug dosing interval and toxicity. This study reported the ability of Leuconostoc dextranicum ATCC 11454 producing nano sized minicells as drug delivery in packaging capacity with antifungal drug named tinidazole to obtain the best condition to achieve a higher degree of Encapsulation Efficiency (EE). By using scanning Electron Microscope (SEM) and transmission Electron Microscope (TEM), morphology of minicells and drug loading in minicells were observed. Remarkably, minicell’s spherical shape and size (less than 400nm) did not change over the time after keeping in buffered saline gelatin (BSG) and packaging with tinidazole. Using Box-Behnken design, the encapsulation efficiency to tinidazole can reach to 90%. Then, tinidazole packaging minicells were orally administered in mice to evaluate the drug absorption and distribution of these minicells with tinidazole administration alone in mice. Tinidazole loading minicells can pass the small intestine faster and prolong action than tinidazole by using high performance liquid chromatography (HPLC) to qualify and quantitative. The study assessed the insoluble antifungal drug named tinidazole delivery of Leuconostoc minicells. Keywords: Leuconostoc dextranicum Minicells Drug absorption Box-Behnken