| dc.description.abstract | Efficient plant regeneration in vitro via direct shoot organogenesis protocol for P.palatiferum is reported in this study. Two sets of experiments were carried out; the first compared different concentrations of BA (0.1, 0.3, 0.5, and 1.0 mg/L) using stem, leaf and petiole excised from 4-6 week-old shoots, while the second set tested the combination of BA, using the concentration gave the best condition for direct shoot organogenesis in the first set, with various concentration of IAA (0.1, 0.2, 0.3 and 0.5 mg/L) and TDZ (0.1, 0.3, and 0.5 mg/L) for shoot multiplication. For shoot regeneration (the first set of experiment), 5 week-old stem explants with BA at 0.3 mg/L concentration was the most effective condition, producing a mean of 3.13 shoots per explant with mean length 0.55 cm. For shoot multiplication (the second set of experiment), MS medium containing 0.3 mg/L BA supplemented with 0.1 IAA was found to be the best medium for its ability of increasing shoots in shoot multiplication than the other medium (increased 1.50 mean number of shoots per explant). The described protocol provides a simple way to regenerate plants through direct shoot organogenesis, which would be large scale production of genetic transformation studies and useful for diversifying our country indoor ornamental plants, increasing the medical plant resources.
Abbreviations: BA – 6-Benzyladenine, IAA – Indole acetic acid, MS – Murashige and Skoog, TDZ – Thidiazuron
Key words: direct shoot regeneration, explant, multiplication, plant growth regulator, P.palatiferum | en_US |
