| dc.description.abstract | This study demonstrates the anti-inflammatory role of Lactobacillus plantarum, a genus of Lactic Acid Bacteria (LAB), on mice eyes infected with pathogenic Pseudomonas aeruginosa via the expression of interleukins IL-6 and IL-8. These interleukins triggered the host’s immune system to secrete neutrophils as well as other leukocytes to be against inflammation caused by pathogens. For the study, Pseudomonas aeruginosa was infected to both corneas on mice for 3 days prior the treatment with probiotic. As inflammation developed, Lactobacillus plantarum was given in oral administration with different dosages of 108, 109 and 1010 daily up to 6 consecutive days. The evaluation of these treatments was carried out by blood component analysis on total leukocytes, neutrophils, lymphocytes and monocytes. Production of interleukin IL-6 and IL-8 was later detected by total RNA isolation and RT-PCR. This result proved the stimulatory effect of Lactobacillus plantarum on infected mice upon enhancing the secretion of such interleukins, thus suggesting a potential therapeutic agent against bacterial infection besides antibiotics in future.
Key words: Lactobacillus plantarum, anti-inflammation, Pseudomonas aeruginosa , corneal infection. | en_US |
