| dc.description.abstract | Intracytoplasmic sperm injection (ICSI) is viewed as a standout amongst the most capable tools for infertility treatment (Q. V. Neri, 2004). Piezo-actuated ICSI technique is applied in mice for many purposes of resarch and was already described step by step (Naoko Yoshida et al.,2007). In this study, we intended to assess the impacts of sperm morphology on the pre-implantation development of ICSI embryos in the mouse. To be more specific, the spermatozoa with various morphologies, including both ordinary and irregular, was collected from the semen of old ICR mice (more than 6 months old) for direct injection to the mature oocytes collected from female ICR mice (10 to 12 weeks old) through ICSI manipulator system. The embryos were cultured in vitro to compare the development of each phase from the zygote stage to the blastocyst stage between embryos fertilized with morphologically abnormal and typical spermatozoa, separately. Besides, the embryos transfer was performed to evaluate the effects of sperm morphologies on post-implantation development. In this study, the results showed that there was no significant difference in term of blastocyst development rate between morphologically normal (1%) and abnormal spermatozoa group (1.3%). However, , the ICSI-derived blastocysts showed poorer morphologies compared with in vivo blastocysts. Additionally, the embryo transfer was recorded as a failure at 18.5 dpc (day post coitus) without the presence of fetus as well as implantation nodes as the sign of successful implantation of the embryos. In conclusion, although morphology of spermatozoa had no effects on the pre-implantation development rates of the embryos from pronuclear formation until the early blastocyst formation after ICSI, it affected the morphologies of the ICSI-derived blastocysts.
Key word: sperm morphology, Intracytoplasmic sperm injection (ICSI), mouse embryos, embryos transfer, pre-implantation development. | en_US |
