Effect of antibiotics in controlling the acute hepatopancreatic necrosis disease of Peneaus Vannamei

Abstract

Outburst of Acute Hepatopancreatic Necrosis Disease (AHPND) happened in three shrimp species (White leg shrimp Litopenaeus vannamei, Black tiger shrimp Penaeus monodon and Chinese white shrimp Penaeus chinensis) are reported the causative agent from Vibrio parahaemolyticus (Vp). In addition, antimicrobials are commonly used in the treatment of infectious diseases in aquaculture industry, however, the extensive using of antibiotics has led to antimicrobial resistance among pathogens in aquatic products. Along with the steady enlargement of the Asian aquaculture industry, many famers use various antibiotics in order to prevent (prophylactic use) and treat (therapeutic use) pathogenic bacterial infections. However, farmers lack of information and knowledge on the usage’s antibiotics when, in the Mekong Deltas in particular and Vietnam in general. The aim of this study focused on effective use of antibiotics as well as the appropriate antibiotic use procedures to minimize the presence of shrimp residues and help limit the mortality cause by AHPD. Minimal Inhibitory Concentration (MIC) test that was the first test in this research, found out that Doxycyclin (1-32 mg/l.) and Ciprofloxacxin (≤ 0,25-1 mg/l,) which were the most susceptible drugs for Vp. Furthermore, the results of MIC test was used for cultured experiments as Prophylactic, Toxicity, and Therapeutic tests. The result showed that Doxycyclin with 3IC dose and 5IC dose used in both prophylactic test and therapeutic test gave lowest mortality on shrimp, with 44,4% and 93,3% respectively. Moreover, Relative Percentage Survival (RPS) of Doxycyclin also gave positive data, up to 87,5% in Prophylactic test and around 1.67% - 6.67% in Therapeutic test. For Ciprofloxacxin, both Prophylactic test and Therepeutic test gave high mortality on shrimp and had low RPS compare to Doxycyclin.