Evaluation of mycofiltration efficiency against shrimp pathogen Vibrio Parahaemolyticus

Abstract

Today, the shrimp production around the globe is threaten by the outbreak of diseases, especially Acute Hepatopancreatic Necrosis Disease caused by Vibrio parahaemolyticus leading up to 100% mortality rate in a short period in shrimp farms. While various methods have been introduced to tackle the disease, high cost and low efficiency are the drawbacks of the current methods. Therefore, applying mycofilter could be a new approach to counteract the phenomenon. In this study, mycofilters made by using Schizophyllum commune, Pleurotus ostreatus and Pycnoporus sanguineus mycelium as they have been proved to have antimicrobial activities against various pathogen. The mycofilters were tested for toxicity prior the challenge test, and have proved to have no toxic toward the white-leg shrimps. The tanks were challenged by adding Vibrio parahaemolyticus suspension at the concentration of 105 CFU ml-1, where Pycnoporus sanguineus and Pleurotus ostreatus shown to have the potential in using as the mycofilter because more than 70% of V. parahaemolyticus was removed and the survival rate ranging from 75 to 90% after submerging the mycofilter into water. However, modifications needed to be applied to this design in order to maximize the potential and improve its performance in future researches. Keywords: Mycofiltration Vibrio parahaemolyticus White-rot, wood-decay fungi Acute Hepatopancreatic Necrosis Disease White-leg shrimp