| dc.description.abstract | Although round spermatid injection (ROSI) can be used as a progressive
assisted reproductive Technology (ART) for treatment of male infertility, however
the successful rate of embryos and full-term development after ROSI is still
significantly lower than that for intracytoplasmic sperm injection (ICSI). In this
study, we succeed in improving the potential of ROSI oocytes developed to the
blastocyst stage and the quality of embryos by treatment of ROSI oocytes with
histone deacetylase inhibitor (HDACi) scriptaid for 6 hours after injection. Our
results found that ROSI oocytes treated with scriptaid prolong duration of histone
H3K9 demethylation in round spermatid origin DNA until the 2-cell stage embryos.
In contrast, high rate of ROSI embryos with high histone methylated at H3K9 in
round spermatid origin DNA was observed at the 2-cell stage embryos in group of
ROSI oocytes without scriptaid treatment. Interestingly, our study also found that
ROSI oocytes treated with scriptaid for 6 hours after injection enhanced newly
synthesized mRNA levels at the 2-cells stage embryos. In conclusion, this study
demonstrated that treatment of ROSI oocytes with scriptaid for 6 hours after
injection improved histone H3K9 modifications, transcriptional activity at the 2-cell
stage embryos, and finally could enhance the successful rate of ROSI embryo
development to the morula and blastocyst stage. Moreover, the quality of blastocyst
in case of ROSI with scriptaid treatment was clearly improved due to the increasing
of the number of inner cell mass (ICM) cells and the total cells in ROSI expanded
blastocyst stage when compared to the blastocyst of ROSI without scriptaid
treatment.
Keywords: Round spermatid injection (ROSI), histone H3K9 methylation, histone
deacetylase inhibitor scriptaid, nascent mRNA, in vitro development of mouse
embryos | en_US |
