Association between SNPs in candidate miRNAs and breast cancer in Vietnamese

Abstract

Breast cancer is a major health problem throughout the world. It is one of the most common cancers among women both in developed and developing countries. To raise the survival of BC patients, genetic factors are used for early diagnosis because they are non-changed factors and give ability of cells to proliferate and metastasize. Since microRNAs target many genes which are vital for development, apoptosis, differentiation, motility and angiogenesis, this study was conducted to investigate the relationships between three SNPs (rs11614913,rs6505162, and rs2910164) located in three miRNAs (miR-196a2, miR-423, and miR-146a) and breast cancer risk among Vietnamese women. By using High resolution melt analysis (HRMA) and Tetra-primer amplification refractory mutation system PCR melting analysis (TAPMA), 600 samples including 300 controls and 300 cases were genotyped. The results showed that the miR-196a2 rs11614913 (C/T) and miR-146a rs2910164 (C/G) polymorphisms were associated with significantly reduced risks of breast cancer (TT vs. CC: OR=0.39, 95% CI= 0.18-0.88, p= 0.022 for rs11614913; and GG vs. CC: OR=0.33, 95% CI=0.13-0.87, p=0.025 for rs2910164 in an additive genetic model). While miR-423 rs6505162 (A/C) was not associated with breast cancer risk in Vietnamese. Our results showed that rs11614913 and rs2910164 could be potential biomarkers for prognosis of breast cancer risk for Vietnamese population. Key words: Breast cancer, miR-196a2, miR-423, miR-146a, polymorphism, high resolution melt, tetra-primer amplification refractory mutation system PCR melting analysis.