The association between candidates SNPS and Vietnamese breast cancer

Abstract

Breast cancer is known as the most common cancer for women, and now has significantly risen in incidence and mortality worldwide including Vietnam. Single Nucleotide Polymorphisms (SNPs) linked to the breast cancer have been identified in Genome Wide Association studies. Among those, three SNPs (rs2046210, rs1219648, and rs3817198) located in ESR1, FGFR2, and LSP1 gene are found to be important markers for breast cancer susceptibility. In this study, the relationships between these three SNPs and breast cancer risk in Vietnamese women were investigated. By using high resolution melting analysis, a sample group of 150 cases/controls for rs1219648 and rs3817198, and 300 cases/controls for rs2046210 were genotyped. Logistic regression association analysis of their genotype and allele frequencies indicated that the differences between case and control populations was not significant and do not affect breast cancer risk for rs3817198 and rs1219648 because their p genotype are 0.739 and 0.102, which are all above the threshold value (p = 0.05). Though, SNPrs2046210 was presented strongly associated with breast cancer risk in both allelic (p = 0.0015) and genotypic (p = 0.0064) analysis. The risk allele A, likes other previous studies, significantly increasing the risk of breast cancer up to 1.425 folds (OR = 1.425, p = 0.0015). However, the genotype AA showed a stronger effect on the risk of breast cancer than the heterozygous AG genotypes. Despite the fact that the sample size was quite small, the statistical power of this study was as expected (58.67%), obviously confirmed the association between SNPrs2046210 and the risk of breast cancer in the Vietnamese population. Keywords: Breast cancer, rs2046210, rs1219648, rs3817198, Estrogen receptor 1 (ESR1), Fibroblast growth factor receptor 2 (FGFR2), Lymphocyte-specific protein 1 (LSP1), HRM, Melting curves analysis, Vietnamese population.