| dc.description.abstract | Cordycep militaris is a medicinal and edible mushroom which broadly used as folk tonic
food. The aim of this study is to test antimicrobial activity, antioxidant activity and
anticancer activity of crude ethanol extract from the examined Cordyceps militaris
fruiting bodies cultivated in Vietnam. Well diffusion method was carried out to evaluate
antimicrobial activity of the extract against Gram-positive bacteria: Staphylococcus
aureus, Bacillus cereus; Gram-negative bacteria: Salmonella typhimurium,
Pseudomonas aeruginosa and Candida albicans. All bacteria were susceptible to the
extract at different concentrations (50 mg/ml, 100 mg/ml, 200 mg/ml), whereas the
tested concentrations shown no growth inhibition on fungi Candida albicans. In terms
of antioxidant activity, the ethanol extract shown weaker scavenging activity on DPPH
(2, 2 diphenyl 1-1-picrylhydrazyl) free radial with SC50 = 1819.87 μg/ml when
compared to standard compound-ascorbic acid (vitamin C) SC50 = 4.67 μg/ml.
Regarding to anticancer activity, the Cordyceps militaris extract exhibited growth
inhibition against various human cancer cell lines including hepatocellular carcinoma
(Hep-G2, IC50 =38.27 ± 5.86μg/ml); breast cancer MCF-7 (IC50 = 41.42 ± 7.56μg/ml);
cervical carcinoma Hela (IC50 =139.57 ± 11.87μg/ml) and non-small cell lung cancer
(NCI-H460, IC50 =190.97 ± 5.73 μg/ml). However, the extract also shown cytotoxicity
on normal cell line (fibroblast) with IC50 = 70.37 ± 14.32 μg/ml which suggested
potential use for cancer treatment from the extract on hepatocellular carcinoma and
breast cancer.
KEYWORDS
Cordyceps militaris; antimicrobial activity; well diffusion method; antioxidant
potential; DPPH scavenging assay; anticancer activity. | en_US |
