dc.description.abstract | Stress-inducible over-expression of adenine isopentenyl transferase - encoding gene (IPT) involving in the biosynthesis of cytokinin (CK), an important phytohormone that plays a significant role in plant development and involves in plant stress tolerance, was proved to have improved drought tolerance ability by present studies. Previous domestic studies indicated that the homologous GmIPT08-transgenic soybean plants showed high physiological potential in drought resistance, which support for the role of GmIPT08 in regulating plant tolerance. In this study, some biochemical traits of GmIPT08-transgenic soybeans were compared to thoseof wild-type (WT) soybeans. According to the results, upon drought stress, the transgenic soybean plants harboring RD29A-GmIPT08had lower level of hydrogen peroxide (H2O2) content. Also under this adverse condition, higher activity levels of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD)found in GmIPT08-transgenic soybean plants indicated that overexpression of GmIPT08 enhanced antioxidant capacity to defend drought induced-oxidative damage. Taken all of these results together, this report firstly showed that GmIPT08 is a remarkable candidate for improving drought tolerance via genetic engineering approach.
Keywords:catalase, drought tolerance, GmIPT08, hydrogen peroxide,peroxidase, oxidative damage, superoxide dismutase, transgenic soybean plant | en_US |