Anti-atopic dermatitis activities of lonicera japonica thunb flavonoids.

Abstract

This research is aimed to determine the effect of Lonicera japonica Thunb flavonoids loaded cream to treat the atopic dermatitis on mice. The purpose of this study was to investigate the suitable percentage of cream containing flavonoids extracted will reduce symptoms of atopic dermatitis significantly. To apply on mice, four cream formulations containing flavonoids extract in different concentration (0%, 1%, 2.5%, and 5%) were topically applied onto the dorsal skin of 2,4-dinitrochlorobenzen-sensitized BALB/C mice once a day during 5 weeks. Cream base and Protopic (Tacrolimus 0.1%) were used as negative and positive controls, respectively. All mice were induced to the assessment of AD-like symptoms including the development of eczematous skin wound, severity of pruritus and histological phenomenon. The results finally indicated that 5% extract- loaded cream was significantly effective in reducing the dermatitis scores from 75% to 37.5% (n=6, p=0.0334) and scratching behavior from 34.1% to 13.6% (n=6, p=0.0481) in week 5 compared to negative control while noticeably reduced the symptoms of parakeratosis, spongiosis, acanthosis. In conclusion, the experiment supported that 5% extract Lonicera japonica Thunb flavonoids – loaded cream did inhibit the effect of AD – like symptom and express the therapeutic potential in the approach to disease treatment. Keywords: 2,4-dinitrochlorobenzene, Lonicera japonica flavonoids, atopic dermatitis, dermatitis score, histological phenomenon.