Introduction of ehretia asperula zoll et mor.adventitious shoots by in vitro culture technique

Abstract

Ehretia asperula (Xa Den) has gained the attention as natural medicine for many chronic diseases especially cancer, because of its oxidative and free-radical-scavenging properties. This study aimed to determine the physiological age and the effect of cytokinins and auxins on inducing adventitious shoots of E. asperula. The appropriate physiological age of E. asperula was 5 weeks. The combination of 0.5mg/L BA and 0.5mg/L Kinetin was appropriate to induce callus and adventitious shoots after 3 weeks. A combination of 0.5mg/L BA and 0.5mg/L IAA was also found to effectively induce callus and adventitious shoots after 3 weeks. It was recommended that the shoots had to be transferred after 5 weeks to avoid aging. Zeatin was not as effective in inducing callus and adventitious shoots as the combinations mentioned above. TDZ was not appropriate for inducing adventitious shoots of E. asperula. The adventitious shoots were short, so they had to be transferred to MS medium supplemented with GA3 to be elongated. Keywords: Ehretia asperula, adventitious shoots, in vitro, BA, Kinetin, IAA, Zeatin, TDZ List of abbreviations cm centimeter L liter mg milligram ml milliliter BA benzylaminopurine Ki Kinetin IAA indole-3-acetic acid Ze Zeatin TDZ Thidiazuron MS Murashige and Skoog