Finding the biological activities of streptomyces from lemon roots
Abstract
The main purpose of this study is to detect the antimicrobial and cytotoxicity activities of Streptomyces sp. isolated from lemon root. The antimicrobial test was performed by well diffusion method, with six test organisms including three Gram-positive (Micrococcus luteus, Staphylococcus sciuri and Staphylococcus aureus) and three Gram-negative (Escherichia coli, Vibrio parahaemolyticus and Pseudomonas aeruginosa). Sulfohodamine B (SDB) assay was used for determining cytotoxicity of Streptomyces sp. crude extract against breast cancer cell line (MCF-7) and liver cancer cell lines (Hep G2). Detection of PKS gene using PCR reaction was also carried out to investigate the ability in producing the potential bioactive compounds of Streptomyces sp. The results showed that Streptomyces sp. exhibited antimicrobial activities on both six pathogens: Micrococcus luteus (0.97 ± 0.17 cm), Staphylococcus sciuri (1.07 ± 0.12 cm), Staphylococcus aureus (1.1 ± 0 cm), Escherichia coli (1.2 ± 0.22 cm), Vibrio parahaemolyticus (0.83 ± 0.05 cm), Pseudomonas aeruginosa (0.93 ± 0.12 cm). Streptomyces sp. also showed cytotoxicity against both MCF-7 (98.44 ± 0.80 %) and Hep G2 (96.03 ± 1.52). With the PKS gene existence, Streptomyces sp. had ability in biological compound production. Therefore, it is worthy to more exploit Streptomyces sp. to investigate the deep biological mechanisms and identify biological compounds.