Relationship of nfxB length alteration and antibioticresistances in clinical pseudomonas aeruginosa isolates

Abstract

Pseudomonas aeruginosa (P. aeruginosa) is one of the common Gram-negative, rod-shape bacteria that cause hospital infection. Moreover, this hospital acquired infection is getting worse because the resistant rate is increasing every passing year. This study investigates the relationship between recently-found 100 bp deletion mutation in nfxB gene and antimicrobial susceptibility profile of clinical P. aeruginosa. 71 isolates collected from 175 military hospital and Ho Chi Minh city Pasteur Institute was tested antimicrobial susceptibility using disk diffusion method and nfxB mutation detected by conventional PCR. The antimicrobial susceptibility profile in this study showed that the resistant rate of Gentamicin and Tobramycin were highest among 11 antibiotics. Moreover, nearly 50% of the collected samples resisted to Fuoroquinolone and Carbapenem groups. This study also found 11 isolates resisted to Colistin, which had not been discovered by any report before in Ho Chi Minh City. Thus, Amikacin, Piperacilin/tazobactam and Colistin were still effective antibiotics to treat multi-drug resistant P. aeruginosa due to their high sensitive rates. However, there were not any isolate positive to nfxB length mutation, so it was not popular in multi-drug resistant P.aeruginosa. 4