| dc.description.abstract | Evaluated the correspondence between EPS as well as components of EPS (carbohydrate and protein) and calcium precipitate process of the ureolytic bacteria. The presence of urease which were split from ureolytic bacteria could be detected by the increasing of pH based on observing the development of color. Then the amount of calcium precipitation and Extracellular Polymeric Substance (EPS) were estimated. As the result shown that, ureolytic bacteria could be detected by the change of color of urea agar from orange to pink. The four strains of bacteria which are V7, H5, V1 and T2 had the best positive reaction with the fasted in changing color. Besides, V1 also had the reaction but weaker in changing of color. In addition, with calcium-precipitating experiment, the highest amount of calcium precipitate belong to the five strains of bacteria which were V7, H5, V1, T2 and TV1 with the percentage from 27.6% to 33.4% of calcium precipitation. Other strains of bacteria have the lower percentage from 13.3% to 22.9%. For EPS extraction, the total amount of EPS of V1, H5 and T2 were 3 strains have the highest amount of EPS from 0.3 to 0.4 g/L, while V7 have the lowest amount of EPS with 0.113 g/L. Additionally, while the calcium precipitate process shown the correlation with amount of carbohydrates (glucose), didn't have any correlation with the amount of EPS and protein. To recapitulate briefly, all of ureolytic bacteria strains have the ability to precipitate calcium. Moreover, it seemed to be no correlation between the amount of calcium precipitation and total EPS.
Keywords:
Ureolytic bacteria
Calcium Precipitation
EPS
EPS Protein
EPS Carbohydrate | en_US |
