| dc.description.abstract | Extracted compounds from basils have been investigated for its effect to reduce the production of prostaglandins E2 via COX-21 in order to evaluate the use of basil in Dengue fever treatment. Docking method is a method that was used to investigating the binding affinity energy of the ligands with the receptor. The result of docking showed that there are 9 compounds in Basil which are accounting for 1% of Basil components have the lower binding energy comparing to arachidonic acid. These compounds are β-Selinene, γ-Muurolene, β-Bourbonene, γ-Gurjunene, β-Eudesmol, β-Bisabolene, (Z)-Muurola-3,5-diene, (Z)-Muurola-4(14),5-diene, -Cubebene. Moreover, there were 2 compounds are β-Selinene, γ-Muurolene have the lower binding energy when comparing to 2 NSAIDs standard drugs. Pharmacophore analysis was used to observe the interaction between the ligands and the receptor. The pharmacophore analysis shows that these 9 compounds bind to COX-2 active site in a similar way which is binding to the side pocket Val-523 position. Only 1 compound is β-Eudesmol had a high inhibitory activity in COX-2 while others showed only hydrophobic interactions. β-Eudesmol had the formation of a Hydrogen bond with Tyr-355 which can prevent the binding of arachidonic acid to COX-2. Therefore, in this study, the Basil did not show the high ability in inhibiting COX-2.
Keywords: Dengue Fever, Dengue Virus, Cyclooxygenase 2, COX-2, Arachidonic acid Basil, Ocimum Basilicum var. thyrsiflora, docking, pharmacophore.
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