Antibacterial activity and bioactive evaluation of plectranthus of amboinicus essentail oil

Abstract

Plectranthus amboinicus is widely known in Vietnam and widely distributed in Tropical Africa, Asia and Australia because of its effective essential oil, which is considered as an ingredient in medicine, food, homemade remedies. The purpose of this study is to figure out the most efficiency extraction conditions to extract essential oil from P. amboinicus. The optimized conditions for MAE were at 110oC, at the ratio of fresh sample to solvent of 1:1.5 (g/ml) in 3 hours. Antimicrobial trials showed that P. amboinicus essential oil was effective against both Gram-positve and Gram-negative bacteria including S.Aureus, S.Mutans, S.Typhi, and E.coli. In terms of total phenolic and flavonoid content, P. amboinicus essential oil had significant amount (TPC = 82.03 ± 0.98 ug/mg) and also exhibited high antioxidant activity (determined by DPPH assay). Keywords: P.amboinicus, hydrodistillation, microwave-assisted extraction, antimicrobial activities, antioxidant activities.