| dc.description.abstract | Among the Pseudomonas species, P. aeruginosa is the most well – known pathogen that causes human infections. P. aeruginosa causes bacteremic pneumonia, endocarditis, meningitis, burn wound infections, and sepsis which are associated with high mortality. Although P. aeruginosa has been isolated from moist, dirt environment and hospitalized patients, only a few studies have been conducted to assess the prevalence and presence of P. aeruginosa on different human body sites. As such, we aim to determine ecological niches of P. aeruginosa on human body and its antimicrobial susceptibility profile. Samples were collected from healthy volunteers following ethical guidelines and isolated using culture methods. Obtained isolates were identified using conventional PCR with primers target for oprL gene, which is specific for P. aeruginosa. Although there was 40.94% (104/254) samples containing bacteria with P. aeruginosa-like morphology, the prevalence of P. aeruginosa in our study population after identification was only 5.11% (13/254). Preferable colonizing site of P. aeruginosa was naris (57.14%; 8/14) following by throat (28.57%; 4/14) and outer ear (14.29%; 2/14). Interestingly, we found a correlation between people with sinusitis and P. aeruginosa colonization at respiratory tract though it is statistically insignificant due to small sample size (30.77%; 4/13, p = 0.119). Furthermore, we observed 69.23% (9/13) of all P. aeruginosa isolates were sensitive to all 10 tested antibiotics including Amikacin (Ak), Gentamycin (Ge), Tobramycin (Tb), piperacillin/tazobactam (Pt), Imipenem (Im), Meropenem (Me), Ciprofloxacin (Ci), Levofloxacin (Lv), Cefepime (Cm) and Ceftazidime (Cz). There were 4 isolates resisted to both Ceftazidime and Cefepime. Among those, only 1 isolate resisted additionally to Gentamycin. In summary, this study observed a prevalence of 5% P. aeruginosa colonization in the community with found no multi – drug resistant isolates.
Keywords:
Antibiotic, antimicrobial susceptibility profile, commensal, ecological niches, Pseudomonas aeruginosa. | en_US |
