| dc.description.abstract | Glinus oppositifolius (L.) Aug. DC belongs to family Molluginaceae and used as traditional medicine for treatment of stomachic, fever, diarrhea, antiseptic, itches and skin disease. G.oppotisifolius contains wide range of phytochemicals, such as Acromatics, Flavonoid glycoside, Triterpenoid saponin, Steroids, Pectin type polysaccharide, Nucleoside, Acylamino acid derivative. This project shows the remarkable amount of total Saponin content derived from Glinus oppotisifolius, 401.803±7.835(%). It also provides the information about the antioxidant activities, antidiabetic activity, and Cytotoxicity activity of extracts, fractions and two predicting isolated compounds, Spergulin A and Spergulacin, which are successful derived from Glinus oppotisifolius. DPPH assay was applied for demonstrate antioxidant activity of crude extract, n-Hexane extract and residue extract, which low percentage of inhibition, 3.793±0.482(%), 5.031±0.635(%), 2.223±0.498(%), respectively. Antidiabetic activity was determined in crude extract, n-Hexane extract, residue extract, ten fractions and 2 isolated compounds by using α-glucosidase inhibitor assay. Fraction 1, F1, shows the highest percentage inhibition, 50.132±0.74, however, fraction 6, F6 shows the lowest percentage inhibition, 2.418±0.255(%). The two isolated compounds have moderate percentage of α-glucosidase inhibition, 14.338±2.709(%) and 14.029±2.490(%) respectively. Cytotoxicity activity of the Glinus oppotisifolius extract and the isolated compounds against human liver cancer cells (HepG2), which was tested using the SRB assay, do not have significant cytotoxicity activity. Specifically, crude extract, n-Hexane extract, residue extract and Spergulin A have negative values of percentage of cytotoxicity while Spergulacin has positive result, 6.18± 6.23(%).
Keywords: Glinus oppotisifolius, G.oppotisifolius, extract, Saponin, Spergulin A, Spergulacin, antioxidant, antidiabetic activity, cytotoxicity, chromatography. | en_US |
