| dc.description.abstract | Although mammalian cloning by somatic cell nuclear transfer (SCNT) has been established in various species, the low developmental efficiency has hampered its practical applications. Treatment of SCNT-derived embryos with histone deacetylase (HDAC) inhibitors can improve their development, but the underlying mechanism is still unclear. As a result, many studies are being conducted to fix these abnormal epigenetic modification occurring in cloned animals. In this study, Trichostatin A (TSA), a potent Histone Deacetylase inhibitor (HDACi) was used to examine its effects on the preimplantation development of cloned bovine embryos. Our experiment investigated the impact of a two steps treatment of TSA on the preimplantation development and zygotic gene activation (ZGA) of cloned embryos. TSA was treated at the first step for 10 hours right after activation and the second step for 10 hours at 48 hours after activation to evaluate the time that embryonic transcription activated. The result showed that two-step treatment improves the quality of cloned bovine blastocyst but had no significant improvement in blastocyst rate. However, a slight increase in the blastocyst formation rate in 2 steps treatment (25%) compared to the control group (18%). Furthermore, there is no symbolic difference in nascent mRNA expression of chromatin at 8-cell stage between two-step treatment with control and one-step group.
Keywords: Trichostatin A (TSA), somatic cell nuclear transfer (SCNT), Histone Deacetylase inhibitor (HDACi), bovine embryos, preimplantation development. | en_US |
