| dc.description.abstract | Fungi have been reported as useful sources of possible biocompounds which are
applied in many fields of human life such as food products, therapeutic,
pharmaceutical purposes and healthy cosmetics. The objective of this study was to
evaluate the antioxidant properties of five species of wood decay fungi Pycnoporus
sanguineus, Flavodon flavus, Fomitopsis ostreiformis, Physisporinus vitreus and
Ganoderma enigmaticum. Five species were cultured in sawdust in 30 days and the
mycelium was collected for ethyl acetate extraction. Folin-Ciocalteau method and
aluminum chloride colorimetric assay were used to determine total phenolic and
flavonoid contents of mycelial extracts. For evaluation of potential antioxidant
activity, scavenging capacity on DPPH radicals, hydrogen peroxide and reducing
power were investigated. F. ostreiformis showed the highest extraction weight with
190.93 mg dry extract weight/g of mycelium weight. The phenolic contents were in
the range of 17.27 – 48.21 mg GAE/g DW, flavonoid contents were in the range of
24.74 – 102.02 mg QE/g DW with the highest values belong to P. sanguineus and
F. flavus, respectively. Especially, F. flavus certified the strongest antioxidant
ability among five species with lowest IC50 value of 290.42 μg/mL and 996.79
μg/mL for in DPPH free radical and hydrogen peroxide scavenging activity,
respectively. P. vitreus and G. enigmaticum expressed the lowest abilities in
antioxidant capacity out of five species however their abilities were good in
comparison with common medicine mushroom. The results demonstrated that the
biocompounds from ethyl acetate extract of fungal mycelium which were cultured
in sawdust could exhibit potential antioxidant activities.
Keywords:
Antioxidant activity
Ethyl acetate
Flavonoids
Phenolics
Sawdust
Wood decay fungi | en_US |
