| dc.description.abstract | Slime molds, a special group of fungus-like protozoans, which possess many novel bioactive compounds. In addition, bacterial exopolysaccharides (EPS) have been proved to have remarkable bioactivities. However, there has been only a few studies conducted with the bacteria associated with plasmodia of slime molds and their EPSs production and bioactivities. In this study, the aim was to investigate bioactivities of the EPSs produced by the bacteria (Enterobacter sp; Stenotrophomonas sp; Achromobacter sp; Alcaligenes sp) isolated from slime molds (Physarella oblonga and Fuligo septica). Regarding antimicrobial activity, at the concentration of 30mg/mL, all EPS samples showed no inhibition toward the tested pathogenic microorganism (Candida albicans Berkhout ATCC 26790). In terms of antioxidant activity, EPS from these bacteria displayed antioxidant activities at the minimum concentration tested 5mg/mL. EPS produced by Alcaligenes sp possessed the highest antioxidant ability (54.08%). Cytotoxicity test by MTT assay found noticeable cytotoxicity effects of all EPS samples at concentration of 1mg/mL toward 2 cancer cell lines including cervical carcinoma (HeLa cell line) and human liver cancer cell line (HepG2cell line). Remarkably, crude EPSs produced by Enterobacter sp could inhibit more than 35% of the cancer cell population in Hela cell line. Moreover, the samples showed no toxicity toward mammalian cell bovine aortic endothelial cells (BAEC cell).
Keyword: Bioactivities, Bacteria, Exopolysaccharides, Fuligo septica, Physarella oblonga, plasmodium. | en_US |
