| dc.description.abstract | Coconut palm (Cocos nucifera L.) is one of the most important commercial crops that is cultivated in multiple tropical regions, mainly grew in the Philippines, Indonesia, India, East and West Africa, the West Indies, etc. Its versatility because an abundantly functional of this palm, mainly for its nutritional and medicinal value. The various products of coconut include tender coconut water, copra, oil and raw material which are essential to both traditional and modern life-style. Makapuno coconut (MAK) (Dừa Sáp) is Tall coconut variety with good characteristics such as big fruit and high weight of fresh meat, which has been prioritized and promoted in Vietnam. Unfortunately, the yield of Makapuno coconut is falling down due to many factors affect to growth and development of Makapuno coconut, such as: senility, breakout of pests and diseases, natural disaster, etc. This lead to high value of coconut nut as well as the need of this type of coconut has been expanding. Therefore, there is great demand for palms selected for high productivity and resistance to disease, and the best strategy for their production is micropropagation through somatic embryogenesis (SE). A protocol which using clonal propagation through somatic embryogenesis has been reported over three past decades, mostly using inflorescence explants and rarely using plumule explant. This research focused on the effect of maturity on formation of embryogenic callus through plumule explant in Makapuno coconut variety. There were three treatments, which differ in time of excising plumule tissue were used to test its effect of callus induction. Results indicated that the most optimized phase for embryogenic callus formation was reported at treatment 2 week after induction. Two of three treatments were cultured on Y3 media before imitating on callus induction, demonstrated a better result in embryogenic morphology than the direct one. In addition, the longer culture stage in Y3, the higher fresh weight was gained. Also, secondary somatic embryogenic was tested for mass production of micropropagation. This result provided better insights of somatic embryogenesis through plumule explant, which might be helpful for future studies. Keyword: Coconut; somatic embryogenesis; callus induction, plumule explant; embryogenic callus; secondary somatic embryogenesis. | en_US |
