| dc.description.abstract | Culture medium is an essential factor that affects directly on the development of
the embryo during pre-implantation stages. In order to improve the quantity
and quality of desired embryo, many treatments have been applied in culture
condition. In this study, to enhance the quality of porcine blastocyst after
culture, a timing assessment was used to evaluate the combination of Ascorbic
acid (AA) and Insulin-Transferrin-Selenium (ITS) when added in IVM medium.
Their effects will be observed based on the maturation of porcine oocytes and
the development of parthenogenetic embryos. 1% ITS will be supplied for the
first 22hrs or 44hrs during IVM with the presence of 50mg/ml AA. After
examining the effect of AA addition, it can be concluded that there are no
significant differences in the oocyte maturation rate between control and treated
group (92.66±2.45%, 93.58±1.66%, respectively), but the blastocyst
production ability was increased after culturing (39.22±2.06%, 46.53±2.03%,
respectively). Likewise, in the study of ITS supplementation, the addition of AA
when combined with or without ITS for the first 22hrs or 44hrs of IVM also
showed no critical distinction among three groups (93.54±2.20%,
95.04±2.10%, 98.39±1.61%, respectively). However, when evaluating AA-ITS
in 20hrs group with AA and AA-ITS in 44hrs groups, it appears to decrease the
quantity of oocytes with non-expanding cumulus cell (2.15±1.08%,
6.46±2.20%, and 4.96±2.10%), hence yielding a higher number of blastocyst
(56.67±1.92%, 46.81±5.01%, and 40.50±5.47%, respectively). To sum up,
the timing assay of antioxidant supplement in the IVM culture medium did not
promote a higher rate for oocyte maturation, but provide positive effects on
developmental competence of parthenogenetic embryo.
Keywords: Ascorbic acid, ITS, IVM, IVD, parthenogenetic embryo. | en_US |
