Analyze The Expression Of A1-Pyrroline 5-Carboxylate Synthase (P5cs) Genes Involved In The Proline Synthesis In Sugarcane (Saccharum Officinarum) Under Drought Stress

Abstract

Drought is one of the very important growth-limiting factors for sugarcane production. Δ¹-pyrroline-5-carboxylate synthase (P5CS) is the rate-limiting enzyme in proline synthesis, and it plays an important role in plant response to drought stress. In this study, P5CS gene segment was inserted into pGEM-T Easy vector and transformed into competent cell E. coli DH5α. The result of PCR detection of segment of P5CS gene was 252 bp in length, the accession number on GenBank was EF620362.1. The RT-qPCR analysis showed that the expression of P5CS of test samples under drought stress was higher when compared with the internal control. The experiment was a factorial based on completely randomized design with three replicates. In sampling time points, the maximum level of mRNA was observed at 2 weeks after stress was applied. For treatment C1 (water withdrawal and then 3-day watering recovery) at 2 weeks after stress induction, the expression of P5CS was almost 1.13-fold higher when compared to the internal control samples with p value < 0.01. In both cultivars, gene expression decreased from 2 weeks to 6 weeks. The results suggested that the P5CS was a stress inducible gene. This gene has the potential to be used for improvement of drought stress tolerance in sugarcane. In conclusion, drought stress application of water deficit was associated partially with enhanced expression of P5CS gene and following proline accumulation. Further research is required for validation and acquiring better understanding of the role of this gene product on plant response to drought stress.