Detecting angelica sinensis extract on antibacterial activities and DNA damage protection from the selected agents

Abstract

Background: Angelica sinensis (Dang Gui) is a well-known Chinese herb that has been utilized for thousands of years in the treatment of feminine illnesses. It is second only to ginseng (Panax ginseng) in terms of popularity, and it is especially well-known for its 'blood tonic' benefits on women. The roots of Angelica sinensis are used in prescriptions for blood replenishment, abnormal menstruation, and other women's disorders. It has also been widely advertised as a nutritional supplement in Europe and America, as well as a health food for women's care in Asia. Objective: To understand the biological activities of Angelica sinensis in order to create a reliable source of information, this project has been carried out. Methods: The decoction method was used to obtain Angelica sinensis extracts. After evaporating the solvents, the residue was dissolved in distilled water and called DWEd used to test for activities. Column chromotography was used to fractionate the total extract of Angelica sinensis and then the fractionated extract was through another column to separate Ginsenoside, Naringenin, Apigenin by checking on thin layer chromatography (TLC). The different concentrations of total extract (100mg/ml; 1000mg/ml ) was used to test the antibacterial activities. For DNA damage protection, total extract (1mg/ml) was used to test on Saccharomyces cerevisiae. Results: In this study, Angelica sinensis have proved the ability of resistance different bacteria (Klebsiella pneumoniae atcc 13883, Salmonella typhi atcc 6539, Pseudomonas aeruginosa atcc 27853, Staphylococcus aureus atcc 6538) and protection Saccharomyces cerevisiae NRRL Y-567 from hydrogen peroxide and hydroxyurea which inhibits DNA replication. Results also showed that the fraction of total extract through column chromatography still has ability to fight bacteria. TLC have shown that sample extract might have apigenin and naringenin, but ginsenoside have not been detected yet in this research. Conclusion: This study has shown that Angelica sinensis extract had DNA protection, antibacterial activities. The study also succeeded in the fraction having antimicrobial activity for further purification. Angelica sinensis extract was also detected apigenin and naringenin that could contribute to these activities. These compounds were not mentioned in this plant before. The study suggests these plant activities should be exploited well for industry.