| dc.description.abstract | Background: Due to reducing the chemical drugs’ negative effects and consequences
outweigh their positive advantages, Traditional Chinese Medicines (TCM) have gained
their attractiveness in recent years. One potential of traditional Chinese Medicine is natural
sources and contains a vat of components.
Purpose: Herba Cistanches, one of the most valuable herbal drugs in TCM, was researched
in numerous pharmacological activities. Since Herba Cistanches contains an abundance of
the components, which are potential to free radical scavenging, antioxidant activity, and
neuroprotective effects, etc. However, the activities depend on the herbal source and extract
processes so, the study tried to screen some important activities such as anticoagulant and
DNA protection ability together with phytochemical components.
Methods: For anticoagulation, the extract was tested using Prothrombin time assay for
blood clotting prevention, and Thrombolytic for breaking up blood clot ability. For DNA
damage protection, the study was tested on Saccharomyces cerevisiae treated with H2O2,
and a DNA damage agent called Hydroxyurea (HU). The antioxidant activity of the
extracts was evaluated by the ability of the extracts to prevent (or) minimize the oxidative
lethal damages induced by H2O2, and HU. For phytochemical screening, the extract was
fractionated and checked for Ginsenoside Rb2 as representative saponin, and Apigenin and
Naringenin as representative flavonoids.
Results: As a result, the appearance of Ginsenoside and Apigenin was confirmed in
extraction, which was not detected in Cistanches before. The thesis also showed the anticoagulation ability by break up the blood-clot and prevent blood-clotting in all the
Cistanches extractions, especially the Ginsenoside fractions, which own highest INR
value. In addition, the DNA damage protection property was proved at the 100μ/mL under
stressing agent 1mM H2O2, and 10mg/mL Hydroxyurea. | en_US |
