Developmental potential of mouse androgenetic embryos during preimplantation development

Abstract

In this study, we examined the developmental potency and the quality of androgenetic ICR mouse embryos during preimplantation development. In order to produce androgenetic diploid embryos, the metaphase II chromosome of mature oocytes was taken out, two sperms head were injected into enucleated oocytes by micro-manipulator system. The survival oocytes were then cultured in the in vitro development culture until the blastocyst stage. Our result showed that the androgenetic embryos developed to morula stage with 5.9% and were blocked at this stage while ICSI embryos could reach to blastocyst stage with 13.9%. We found that during the preimplantation developmental time, the survival rate of ICR oocytes after enucleation and injection is quite low and the developmental rate of androgenetic embryos was obviously lower than that of ICSI embryos. This study also showed that creating the androgenetic embryos using manipulator is timeconsuming process required the high technique skill and ICR mouse strain may not was suitable of generating androgenetic embryos.