| dc.description.abstract | Turmeric (Curcuma longa L.) is renowned for its medicinal properties and its
contribution to the food industry. However, this perennial herb is prone to diseases
and requires a great deal of time to produce a large number of rhizomes, causing
a shortage of initial material for propagation. Therefore, an alternative approach
through micropropagation of shoot tips was applied for obtaining a large quantity
of disease-free planting material. In this research, calcium
hypochlorite was studied at 2 concentrations and various durations as a
replacement for sodium hypochlorite for the surface sterilization. Also, the present
study was conducted to examine the effect of BAP in combination of NAA on
multiple shoot clusters formation. The effect of combination of BAP and three kinds
of auxin (IAA, IBA, NAA) on the multiplication of shoot were investigated in this
study. The result showed that the effective protocol for sterilization was Calcium
hypochlorite at 5% in 30 minutes, followed by 0.1% mercuric chloride in 10
minutes with an addition of three drops of Tween 20. Besides, a combination of 3
mg L-1 BAP and 0.5 mg L-1 NAA induced the highest number of multi-shoot clusters.
Finally, an addition of 0.3 mg L-1 IAA in the MS medium supplemented with 3 mg
L-1 BAP induced the highest multiplication rate and can be considered a potential
media for shoot multiplication | en_US |
