| dc.description.abstract | In this research, the utilization of ethyl lactate as a green solvent for the
carotenoid extraction from dried Drumstick leaves was evaluated under
ultrasound-assisted conditions. Additionally, the efficiency of liquid-liquid
leaching with water in non-evaporative elimination of solvent from the crude
extract was also under investigation. Drumstick leaves are potential source of
nutrients and bioactive compounds, especially carotenoids which are widely
applicable in food processing and other industries. Ratio between sample and
solvent (0.5:10, 1:10, 1.5:10), ultrasonication temperature (30°C, 45°C, 60°C,
75°C) and duration (15 min, 30 min, 45 min) were three variables for carotenoid
extraction; ratio between the crude extract and water (1:1, 1:2, 1:3), number of
leaching times (1 time, 2 times, 3 times) and temperature for leaching (30°C,
40°C, 50°C) were three variables for non-evaporative elimination of solvent from
the crude extract. The results showed that the mentioned factors significantly
affected the total carotenoid yield extracted from dried Drumstick leaves. 2.529
0.053 mg beta-carotene equivalent/ g dry weight of Drumstick powder was
obtained under 1:10 ratio between sample and solvent, 60°C of ultrasound
temperature and 30 min of duration. In addition, the solvent cleanliness in the
final extract was influenced significantly by the evaluated parameters. The
amount of residual solvent in the final extract was 3.08 0.03 % and carotenoid
loss after leaching was 10.92 0.13 % achieved under 1:3 ratio between crude
extract and water, 2 leaching times and 40°C of leaching temperature. | en_US |
