Mycofiltration Using Schizophyllum Commune And Pleurotus Ostreastus Against Vibrio Parahaemolyticus In Penaeus Vannamei (White Leg Shrimp) Farming

Abstract

Nowadays, shrimp production is one of the biggest industries in Vietnam and the government is pushing to expand it toward environmentally sustainable development. This research will provide scientific data about inhibiting Vibrio Parahemolyticus (bacterial pathogen) causing Early Mortality Syndrome/ Acute Hepatopancreatic Necrosis Syndrome (EMS/AHPND) in shrimp farming and the feasibility of using mycofiltration method in shrimp aquaculture. In this project, 2-different fungi species Schizophyllum commune and Pleurotus ostreatus are intended to apply for testing and verifying the feasibility and efficiency in percentage of bacteria removed when facilitating mycofilter treatment. According to Long M. Tran and Long H. Dinh, International University’s bachelors, Schizophyllum commune, Pleurotus ostreatus and Pycnoporus sanguineus were testified but still being included some mistakes and uncontrollable difficulties when running experiment. In this research, experiment applied into mycofiltration with the mass of mycelium in hessian bags (around 170g - 200g) in each independent tanks and water circulation flowing through mycelium in filter then dropping down into each replicate. At first cycle, applying Schizophyllum commune in infected tanks made a profound contribution in prolonging shrimp life span compared to other treatments. Meanwhile, the second cycle showed that infectious shrimp applying Pleurotus ostreatus gave significant effects on mycelium feasibility inhibiting Vibrio Parahemolyticus. After 25 hours, bacterial-infected group recorded survival rate less than 10% while two designs treated with fungi were remained more than 30%. The mortality rate of infected shrimps, however, is still significantly high and some procedures need modifying for better results. Moreover, scales of this project are suitable for only experimental shrimps which the minimal number is applied in each treatment, giving no obvious definite in mycofiltration implementation in big shrimp industry.