Detection And Quantification Of Amoxicillin Antibiotic Resistant Bacteria And Gene In Urban Surface Water

Abstract

The environmental spread of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) is a serious health protection problem worldwide. This study aimed to detect and quantify amoxicillin antibiotic resistant bacteria and genes from urban surface water in Vietnam. The level of Amoxicillin resistant genes was determined using the real-time PCR method. PCR and sequencing analysis showed that the predominant β-lactamase genes were blaSHV followed by blaCTX-M and blaTEM. The results of this study show a high prevalence of Amoxicillin resistant bacteria in surface water. These findings can help to control Amoxicillin antibiotic resistance in the environment to improve water security.