| dc.description.abstract | Lipids, specifically fatty acids and monoglycerides, have emerged as new and
potential objects due to their pharmaceutical properties. Slime molds are a group of
fungus – like protozoans. One of their trophic stages is plasmodium. In liquid
medium, plasmodia are fragmented into microplasmodia. To the best of my
knowledge, there was only one report on bioactivities of plasmodial lipid extract,
and there has not been study on bioactivities of microplasmodial lipid extracts from
slime molds. Therefore, the main objective of this project was to evaluate
bioactivities of lipids extracts of microplasmodia of Physarum polycephalum. Lipid
extracts from plasmodia of P. polycephalum and Physarella oblonga were also
included for comparisons.
Microbial activities of the lipid extracts were evaluated using well diffusion
assay. Both the lipid samples isolated from plasmodia and microplasmodia of P.
polycephalum showed weak inhibitory activities toward Staphylococcus aureus at
the concentration 100 mg/mL and displayed no activity against Pseudomonas
aeruginosa and Candida albicans. Regarding antioxidant activity, the highest DPPH
radical scavenging rate was exhibited by Phy. oblonga plasmodial lipid sample,
followed by that of microplasmodial lipids of P. polycephalum. At the concentration
of 150 mg/mL, these samples accounted for 74.36% and 67.95% of the DPPH
radical scavenging activities, respectively while P. polycephalum plasmodial lipids
showed the inhibitory rate of only 59.69%. In addition, cytotoxicity of the extracts
done by SRB assay showed that at the concentration of 100 µg/mL, micoplasmodial
lipids of P. polycephalum could inhibit 20,62% on Jurkat cell, which is significantly
higher than other samples.
Generally, this study generated a first set of data on bioactivities of crude
lipids extracted from microplasmodia of P. polycephalum. The data suggested that
the microplasmodial lipids would be worth further investigation in cytotoxicity
toward Jurkat cells. | en_US |
