Study The Culture Conditions Of Mycelial Growth Of Domestic Cordyceps Cicadae

Abstract

Cordyceps cicadae is a medicinal mushroom, which had been widely used by Chinese in traditional treatments including cure for fever, typhus, vision improvement and protection of renal function, etc... Recent studies also indicated that it can be used as replacement for Ophiocordyceps sinensis. Nowadays, Cordyceps cicadae had been studied to reveal the chemical components and mechanisms of action of its biomedical capacity, along with several strains of Cordyceps cicadae that were discovered in Vietnam. Because of the increase in need of Cordyceps cicadae production, optimal conditions for cultivating Cordyceps cicadae was essential to carry out. In this study, four strains of Cordyceps cicadae were taken for cultivation in order to choose the best strain as well as suitable conditions for growing. Therefore, the best strain for cultivation was strain AHTP-F0002, the suitable medium was Schizophyllum (Mushroom) genetics Complete Medium plus yeast extract(MCM). The optimal temperature and pH for culturing mycelium were found at 30oC and 7.0, the collected dried biomass yielded at 0. 249g/40ml and 0.19g/40ml, respectively. Among all carbon and nitrogen sources that had been tested, sucrose and peptone resulted in the best dried mycelial biomass at 0.208g/40ml and 0.205g/ml, respectively. In conclusion, the results of this study could be applied for various processes that related in biomass production of Cordyceps cicadae strain AHTP-F0002.