| dc.description.abstract | S.Heptaphylla belongs to the family Araliacae. The purpose of this study is to investigate
antibacterial activity, antioxidant activity and cytotoxicity of two crude extracts from
stem bark of Schefflera Heptaphylla, namely dichloromethane extract and methanol
extract. Disc diffusion method was employed to identify the minimum inhibitory
concentration (MIC) of the crude extract. Regarding the antibacterial activity, it is
observed that Schefflera Heptaphylla crude extracts were weak in the inhibition of two
bacteria strains, Escherichia Coli (gram negative) and Staphylococcus aureus (gram
positive), and the weakest inhibition occurs at 500 µg/ml on S.aureus. 1-diphenyl 2-
picrylhyorazyl (DPPH) assay was used to test the antioxidant property of the crude
extracts with various concentrations (25µg/ml to 400 µg/ml). The result showed that
dichloromethane extract had IC50 value of 154.40 µg/ml, which is higher than that of
methanol extract (712.00 µg/ml). Compared to Ascorbic Acid, the crude extract was
reported to be somewhat weaker with IC50 of 154.40µg/ml. The cytotoxicity of crude
extracts were tested on chronic myeloid leukemia (K562), chondrosarcoma (SW1353)
and liver carcinoma (HepG2), determined by using MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-
diphenyltetrazolium bromide (MTT) assay and reported in terms of cell viability. The
dichloromethane and methanol extracts had a significant cytotoxicity against chronic
myeloid leukemia with IC50 value of 0.75 and 1.02 µg/ml, respectively, and
chondrosarcoma cells with IC50 value of 7.29 and 8.80 µg/ml, respectively. | en_US |
