| dc.description.abstract | One of the most challenging milestones in tissue culture of coconut (Cocos nucifera
L.) is soil acclimatization due to the variable conditions between in vitro and ex
vitro stages. The present study aims to investigate efficient conditions for rooting
and soil acclimatization. Prior to the ex vitro stage, the root system was induced
and hardened, using various concentrations of 1-naphthaleneacetic acid (NAA),
i.e. 0, 30, and 60 µM. Well-developed seedlings were transfered to a mini growth
chamber (MGC) for ex vitro acclimatization. Three concentrations of Y3 basal salts
were investigated: nill, half-strength and full-strength. Furthermore, a comparison
was made between 1-naphthaleneacetic acid (NAA) and indole-3-butyric acid
(IBA) to elucidate their effect on ex vitro growth of the seedlings in the MGC.
Results showed 30 µM NAA was likely to promote leaf and root formation, while
60-µM NAA enhanced shoot and root elongation. In terms of seedling survival,
half-strength Y3 basal medium was demonstrated to be more suitable. This study
confirmed that the presence of Y3 in MGC was necessary for the survival of
seedlings during the first two weeks of the ex vitro stage. It was also found that
IBA can help boost up the survival rate of seedlings (up to 73%). This study
provided important insight into soil acclimatization of in vitro coconut seedlings
and will open up new opportunity for efficient transfer of tissue-cultured plantlets
into soil for palm species. | en_US |
