| dc.description.abstract | Purification of nucleic acids is an essential procedure for most experiments in
molecular biology because the presence of impurities can interfere with such
molecular applications as restriction digestion, ligation, cloning, labeling
sequencing, PCR and RT-qPCR. In order to solve this problem, several methods
for purification of DNA fragments have been developed, such as those utilizing
phenol-chloroform, paper strip and magnetic particles (for aqueous solutions);
other methods include purifying DNA from fragments of agarose gel, known as the
freeze-squeeze method. This method has been widely used for DNA purification
but has the disadvantage of using low-melting point agarose or requiring high
concentrations of DNA samples. Based on the above mentioned considerations and
our needs for purifying and analyzing small fragments of DNA or molecules.
Moreover, there are many kinds of buffer which are used as commercial kits
buffers such as KI, Guanidine Hydrochloride (GuHCl), Guanidine Thyocynate
(GuSCN), etc. In fact, it is important to emphasize that this thesis is to compare
these buffer effects to the task. After go through many steps we gained the result
that KI and GuSCN had as similar quality as commercial buffer. Although, KI
always had the outstanding result when measured by NANODROP the out-come
expressed by gel electrophoresis is more reliable than by NANODROP
measurement. Therefore, GuSCN is the best one. | en_US |
