dc.description.abstract | Response surface methodology using Box-Behnken design was employed
to optimize and determine the effects of three independent variables of
extraction process, namely time in microwave (20 to 60 s), temperature
in ultrasound (45 – 75oC) and time in ultrasound (40 to 80 min) on the
total chlorophyll content and antioxidant capacity. The results indicated
that the selected extraction variables had significant effects on the total
chlorophyll content and antioxidant capacity. The optimal extraction
condition was determined as following: time in microwave of 43 s,
temperature of 55oC, and time of 65 minutes. At this condition, the total
chlorophyll contents of the extract were 8.55 mg/g dry weight and its
antioxidant capacity as Ferric Reducing Antioxidant Power (FRAP) Assay
capacity was 0.39 mmol TE/g dry weight. As the result, the extract of
Thai basil leaves exhibited fairly high antioxidant capacity and total
chlorophyll content as compared to other medicinal plants. Therefore, the
extract of Thai basil leaves may be used as a source of bioactive
compounds for health benefits. | en_US |