| dc.description.abstract | Phycocyanin is a pigment-protein complex synthesized by blue-green microalgae
Spirulina platensis, which is used mainly in pharmaceutical and food industry as
their antioxidant and anti-cancer activities. In the world, the phycocyanin
content in spirulina has been extracted at 7% while the figure for domestic
enterprises is about 3.5-4%. In Vietnam, the highest content of phycocyanin
extracted was lower than 5%. The limitation came from the extraction method
as the cell wall of spirulina is composed of four resistant layers. The objective of
this study was to obtain the highest yield of phycocyanin under different
extraction methods combined with different solvents. Methods used in this study
for phycocyanin quantification were using a spectrophotometer at wavelengths
652nm and 615nm. Phycocyanin is an important commercially available blue
food colourant. This report studied various phycocyanin extraction methods as
homogeneous, ultrasound, cold maceration and freeze-thaw extraction methods
from dry biomass of Spirulina platensis. Three different solvents i.e. distilled
water, 1.5% CaCl2 (w/v) solution and 0.1M phosphate buffer were applied as the
extraction medium. As a result, ultrasound extraction method with 1.5% CaCl2
(w/v) solution provided vivid blue colour, a higher amount of phycocyanin (70.08
mg/g) higher than previous studies, high of purity (3.40), total phenolic content
(182.05%), and maximum antioxidant activity as compared to other extraction
methods. | en_US |
