| dc.description.abstract | In this study, the microwave-assisted extraction variables were optimized using a
Box-Behnken design of response surface methodology for the optimal values of
DPPH antioxidant capacity and total phenolic content of Thai basil (Ocimum
basilicum var. thyrsiflora) leaves. The independent variables were ethanol content
(10-70 %), microwave power level (80-800 W), and extraction time (2-12 min).
The results showed that the optimum conditions were analysed with 37% ethanol
in water, microwave power of 435 W for 7 min. The value of R2 and adjusted R2
were > 0.98, indicating the good fitting of the models. These optimum conditions
were applied to obtain the predicted antioxidant capacity of 302.77 μmol Trolox/g
dry weight of sample and the total phenolic content of 41.64 mg GAE/g dry weight
of sample. Meanwhile, the experimental values were 300.15 ± 2.31 μmol Trolox/g
dry weight of sample and 39.41 ± 0.87 mg GAE/g dry weight of sample,
respectively. There were no significant differences between the predicted and
experimental values. This implies that the experiment design of Box-Behnken
design and response surface methodology is suitable in optimization of extraction
process. | en_US |
