Investigation Of Hydroxyapatite Incorporation On Characteristics Of Self-Crosslinking Hydrogel Based On O-Carboxymethyl Chitosan And Oxidized Xanthan Gum For Bone Tissue Regeneration.

Abstract

In situ crosslinking hydrogels have increasing rapidly as an interesting topic in the field of biomaterials, particularly in the context of the newly emerging 3D bioprinting technique. This is due to the fact that researchers are hopeful that these hydrogels will soon be able to satisfy the massive demand for organ and tissue transplants, even the more complex ones. In this study, a novel hydrogel system via self-crosslinking was fabricated and analyzed. It was an imine crosslinking hydrogel that was based on N,O-carboxymethyl chitosan (NOCC), oxidized xanthan gum (OXG), and low-cost domestic component. Different hydrogel fabrication parameters, such as varying the temperature and making adjustments to the volume ratio of the solvent, were studied. On the modified materials, a Fourier transform infrared spectroscopy (FT-IR) analysis and an assessment of the precursor solution viscosity were carried out. On the hydrogel samples, a scanning electron microscope (SEM) was used to analyze the cross-sectional surface morphology, equilibrium swelling degree, and in vitro degradation behavior. The FT-IR measurements provided conclusive evidence that every component had been altered. The study of reaction efficiency, solution viscosity, hydrogel morphology, swelling, and degradation behavior revealed how NOCC or OXG component contributed towards hydrogel characteristics. Additionally, the study revealed that the beneficial effect of raising total polymer fractions should be considered despite the difficulty of homogeneous mixing. It is difficult to graft as well as determine the carboxymethyl group onto the chitosan backbone while synthesizing hydrogel samples; however, the results obtained from the raw materials and hydrogel samples are still reliable, and they reached the parameters are satisfactory showed desired hydrogel properties for applications in the field of tissue engineering. Overall, gaining a fundamental comprehension of the process of hydrogel modulation will assist in the production of a hydrogel composite that is superior in terms of its performance in specific applications.