Enhanced Burn Wound Healing In Mice Model Using Low-Level Laser Therapy (Lllt) In Combination With Adjuvant.

Abstract

Burn is an injury to the skin caused by heat or external agents (electricity, chemicals, radiation). Burns are curable, mild to moderate burns (e.g., boiling water burns, sunburn) can be treated at home or resolved on their own but must be combined with treatment and favorable conditions. For severe burns, the structural layers of the skin are almost destroyed, the healing process will become extremely complicated and difficult. Burns cause bad effects on damaged skin and can cause thick scars that are unsightly. Finding a treatment method to shorten the treatment time, increase the regeneration of new tissue, and at the same time have widespread popularity and easy to use, is a necessity. Recent studies show that laser has a good effect on wound healing, minimizing treatment time as well as complications during treatment. This study proposed a method of treating third-degree burns on a mouse model based on the biological effects that occur when laser photons affect the tissue, which are tissue regeneration, vascular proliferation, aid in wound healing, and in combination with suitable excipients to shorten the healing time and minimize other problems (such as infection, necrosis) that may occur during treatment. Rats are scalded with an automatic burn generator (with parameters set for third-degree burns, the temperature is 80 degrees Celsius, and the pressure is 40N for 15 seconds). The laser uses a pulse combination of 650nm, 780nm, and 830nm wavelengths. Excipients are turmeric cream, which helps to support the wound from becoming infected, and at the same time reduces the formation of scars after the healing process. The treatment process took place within 2-3 weeks, with the following dosage: Laser was applied 4 times a week in 2 weeks with 7 treatment sessions, turmeric cream applied daily until the end of the treatment sessions. The results showed positive results in the treatment of burns through the combination of Low-level laser therapy and excipients (turmeric cream). The wound is dry, not infected, the skin around the wound is hairy, and the wound heals well.