Fabrication And Characterization Of A Potential Hemostatic Dressing Made Of Bacterial Cellulose And Chitosan Oligosaccharides

Abstract

In recent years, there has been a growing interest in using bacterial cellulose for biomedical applications thanks to its higher purity and more desirable properties. Regarding hemostatic application, however, most studies on NO2-based oxidized bacterial cellulose (OBC) have focused on its use as a coating agent on another polymer substrate. In this study, we aimed to fabricate a hemostatic dressing of OBC itself by oxidizing an intact bacterial cellulose pellicle and investigating different reaction parameters, including pellicle dryness and oxidation period. Macroscopic observation showed that partially dried pellicles maintained better structural integrity than completely dried pellicles. Fourier-transform infrared spectroscopy confirmed the change in the chemical composition of all OBC samples through the appearance of carboxyl groups. Although scanning electron microscope (SEM) images revealed no significant difference in cellulose fiber morphology between OBC membranes and pristine membranes, the tensile strength of all OBC samples was lower than the unmodified one. Along with the Chitosan oligosaccharides (COS) loading to improve the anti bactericidal of the membranes. The antibacterial property was evaluated by using the agar disk diffusion method against gram-positive and gram-negative bacteria. Moreover, the study also investigated the hemocompatibility of materials to show that the OBC-COS membranes could be a promising material for application in excessive bleeding treatment with further improvement and exploration