| dc.description.abstract | A multi-copper enzyme called tyrosinase is essential to produce melanin. However,
the overproduction of melanin can be unsightly, so tyrosinase inhibitors are
extensively studied today, especially as inhibitors in nature. In this study, the
efficacy of a combination of Camellia Sinensis (green tea) and Oryza Sativa (rice
bran) to inhibit the activity of tyrosinase was evaluated. While Oryza Sativa was
extracted with EtOH using the ultrasonic method, Camella Sinensis was extracted
using the Soxhlet method. The weight of sample obtained after C.sinensis and
O.sativa extraction was 2.52g ± 0.2 and 5.08g ± 0.22, respectively. After
extraction, samples were obtained to use TLC, LC-MS, and GC-MS techniques to
identify and quantify the components. Consequently, whereas the rice bran sample
included anthocyanins and fatty acids, the green tea sample contained several
polyphenol components including GC, EGC, EC, EGCG, and ECG. Both the
extracted samples and their combination showed tyrosinase activity inhibition.
Due to the green tea extract's antioxidant protection, the combination of the two
extracts at 100 ppm achieved the maximum percentage inhibition at the ratio
C.sinensis:O.sativa (5:5) of 56.76% ± 0.15. The IC50 value of the ratio 5:5 was
66.30ppm, which was quite close to that of Kojic acid (51.35ppm). So, it had
potential to replace kojic acid as an inhibitor of tyrosinase in cosmetics. The results
of an evaluation of the tyrosinase inhibitory activity of two serum products (one
water-based and the other oil-based) with the ratio C.sinensis:O.sativa (5:5) were
18.7% ± 0.38 and 22.5% ± 0.41, respectively. | en_US |
