| dc.description.abstract | In recent decades, the issues relating to improving and expanding some medical tests,
especially diagnostic cost, implementation process, and supply chain management are
becoming more and more interesting. To deal with these problems, we tend to apply
an efficient method to replace common methods, which is the use of saliva as a
diagnostic fluid. The main positive feature of our approach is to use saliva instead of
nasopharyngeal or nasal swabs, which enables non-invasive sampling without the
need for trained staff. This advancement makes this material a safer source because of
the protection of healthcare workers from being inadvertently exposed to potentially
infectious droplets. In addition, we shortened the testing process by (1) not using
universal transfer medium at sample collection, (2) the isolation and purification
process were skipped, and (3) testing specimens in just one-step quantitative reverse
transcription PCR (RT-qPCR) assay. During the project, several downsides of
running real-time PCR on saliva samples had been successfully overcome. Pretreating the specimen with temperature and additives could overcome some
inhibitTheThese above arguments can prove that saliva can be identified as a potential
biomarker, besides noninvasive alternatives to swabs large-scale testing. Therefore,
RT-qPCR assay on saliva is a flexible and inexpensive option to help improve
diagnostic capacity in clinical as well as cope with future pandemics. | en_US |
