Evaluation Of Antioxidant And Anti-Hyperuricemic Activities Of Aqueous Extract From Ensete Glaucum (Roxb.) Cheesman Seeds

Abstract

Ensete glaucum (Roxb.) Cheesman (EG) is a dietary and medicinal plant, which Vietnamese people have been utilizing to treat many diseases; however, there is little publication about its therapeutic potents. The present study attempted to investigate the evidence of antioxidant and anti-hyperuricemic activities of aqueous extract of Ensete glaucum seed (AEGS) via in vitro and in vivo models. The antioxidant effect of AEGS was preliminarily screened via quantification of total polyphenol content (TPC), total flavonoid content (TFC), DPPH free radical scavenging, ABTS radical cation scavenging, reducing power, and xanthine oxidase inhibitory (XOI) activities. The antihyperuricemic effect of AEGS given orally (200 and 400 mg/kg/day for a week) was examined in hyperuricemic mice induced by the combination of intraperitoneal and intragastric administration of oxonic acid potassium salt and xanthine, respectively. According to phytochemical quantification result, the total polyphenol and flavonoid contents contained by AEGS were 47.19 mg Gallic Acid Equivalent/g d. w. and 18.00 mg Quercetin Equivalent/g d. w., respectively. Besides, AEGS can scavenge DPPH with IC50 129.71 and ABTS with IC50 50.70 µg/mL, as well as its reducing power was experienced with EC50 measuring at 265.23 µg/mL. Xanthine oxidase activity was significantly inhibited in vitro when AEGS was present; IC50 in the XOI assay was 483.71 µg/mL. The obtained results revealed that AEGS had the capacity for antioxidation in multiple aspects. Regarding the antihyperuricemic activity, AEGS was not evaluated as a promising alternative medicine for the treatment of hyperuricemia in the mouse model according to the mechanism of allopurinol since it did not reduce serum uric acid levels compared with untreated groups. Therefore, applying EG seed in patients with hyperuricemia should be carefully considered.