| dc.description.abstract | Previous study found that Physarum polycephalum (P.polycephalum) microplasmodial
would be an ideal species for functional food development of which the biomass
obtained from glucose or sugarcane powder-based medium displayed a high
nutritional value. Since fresh sugarcane juice is a more affordable substrate compared
to glucose, thus, the main objective of this study was to evaluate the effects of the
composition and cultivation condition on P. polycephalum growth and the nutritional
value of the biomass including protein and amino acid profiles in fresh sugarcane juice
based medium.
Optimization of the sugarcane-based media components by Response Surface
Methodology (RSM) showed that sugarcane concentration had a significant effect on
both biomass production and protein content. The optimal medium consisted of 50 ml
fresh sugarcane juice (equivalent to 37.5 g/L reducing sugars), 15 g/L tryptone, and
1.0 g/L yeast extract. This medium yielded 22.682 g/L dry cell weight with 16.84%
protein after five days of cultivation. These are significantly higher than the amount of
biomass (13.86 g/L) and protein content (14.88%) obtained from the previously
reported optimal sugarcane powder medium.
Quantification of amino acid profiles by HPLC-UV showed that the total amount of
amino acids in the optimal sugarcane juice medium was higher than that of sugarcane
powder medium. Remarkably, some essential amino acids are also significantly
increased, such as isoleucine (8.45 mg/g), leucine (11.78 mg/g), lysine (10.45 mg/g),
tryptophan (6.74 mg/g). Aspartate was found to be the most predominant amino acid
with 17.05 mg/g.
This study found that sugarcane juice is an ideal replacement for glucose and/or
sugarcane powder. Biomass production from this substrate is not only cheaper, but
also better in term of yielding higher contents of the essential amino acids. | en_US |
